How to Make an Origami Chimera

Abstract

Structural DNA nanotechnology^1,2,3,4 and the DNA origami technique⁵, in particular, have provided a range of spatially addressable two- and three-dimensional nanostructures^6,7,8,9,10. These structures are, however, typically formed of tightly packed parallel helices^5,6,7,8,9. The development of wireframe structures^10,11 should allow the creation of novel designs with unique functionalities, but engineering complex wireframe architectures with arbitrarily designed connections between selected vertices in three-dimensional space remains a challenge. Here, we report a design strategy for fabricating finite-size wireframe DNA nanostructures with high complexity and programmability. In our approach, the vertices are represented by n × 4 multi-arm junctions (n = 2–10) with controlled angles, and the lines are represented by antiparallel DNA crossover tiles¹² of variable lengths. Scaffold strands are used to integrate the vertices and lines into fully assembled structures displaying intricate architectures. To demonstrate the versatility of the technique, a series of two-dimensional designs including quasi-crystalline patterns and curvilinear arrays or variable curvatures, and three-dimensional designs including a complex snub cube and a reconfigurable Archimedean solid were constructed.

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Acknowledgements

This research was partly supported by grants to H.Y. and Y.L. from the National Science Foundation (nos. 1360635 and 1334109), the Army Research Office (no. W911NF-12-1-0420) and the National Institutes of Health (no. R01GM104960). H.Y. was supported by the Presidential Strategic Initiative Fund from Arizona State University. The authors thank M. Madjidi for proofreading.

Author information

Affiliations

1. The Biodesign Institute and the Department of Chemistry and Biochemistry, Center for Molecular Design and Biomimetics, Arizona State University, Tempe, 85287, Arizona, USA

   Fei Zhang, Shuoxing Jiang, Yan Liu & Hao Yan

2. Department of Chemistry, Purdue University, West Lafayette, 47907, Indiana, USA

   Siyu Wu, Yulin Li & Chengde Mao

Contributions

H.Y., Y.L. and F.Z. conceived and designed the experiment. F.Z., S.J., S.W. and Y.L. performed the experiments. F.Z., S.J., S.W. and Y.L. analysed the data. All authors discussed the results. All authors contributed to the writing the manuscript.

Corresponding authors

Correspondence to Yan Liu or Hao Yan.

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Competing interests

The authors declare no competing financial interests.

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Cite this article

Zhang, F., Jiang, S., Wu, S. et al. Complex wireframe DNA origami nanostructures with multi-arm junction vertices. Nature Nanotech 10, 779–784 (2015). https://doi.org/10.1038/nnano.2015.162

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• Received: 19 March 2015

• Accepted: 23 June 2015

• Published: 20 July 2015

• Issue Date: September 2015

• DOI : https://doi.org/10.1038/nnano.2015.162

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How to Make an Origami Chimera

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